The Center implements scientific projects based on high-throughput technologies in the areas of genomics and transcriptomics.

Test equipment:

• next generation sequencers - MiSeq and NextSeq (Illumina),
• microarray scanner - HiScan (Illumina)
• Real-time PCR - CFX384 (BioRad), CFX96 (BioRad), MiniOpticon (BioRad)
• stations for automatic reaction preparation (biorobots) - Bravo Liquid Handling System (Agilent)
• apparatus for automatic nucleic acid isolation - Maxwell 16 (Promega)
• DNA fragment selection apparatus - BluePippin (Sage Science)
• Luminometer - Glomax (Promega)
• DNA fragmentation kit - Bioruptor (Diagenode)
• freezers -80 ° C

Commercial research:

NGS sequencing:

• Identification of causative variants in monogenic diseases
• Detection of structural variants
• Copy Number Variation (CNV) detection
• Detection of somatic mutations in cancer

Microarray techniques:

• Genotyping
• Methylation analysis
• Analysis of cytogenetic changes

Data Analysis:

• Pipelines for data analysis:
  • DNA-Seq:
    • Variant calling – BWA/Bowtie2, GATK, GEMINI
    • Copy number variations (CNVs) – XHMM
    • Identification the causative genes for rare disorders – PhenGen
• RNA-Seq:
  • Variant calling – STAR, TopHat2, GATK
  • Differential analysis of microRNA – SHRiMP, DESeq2
  • Gene fusion detection – TopHat2, ChimeraScan, Chimera
• Metagenomics – Amplicon 16S
  • Biodiversity analysis – QIIME
  • Taxonomic analysis – QIIME
  • And other R analytical scripts prepared at the Center

Research methods used:

Basic molecular biology techniques are widely used in the laboratory:

• cell cultures,
• nucleic acid isolation,
• evaluation of their quantity and quality,
• microarray methods:
  • genotyping,
  • methylation assessment
  • new generation sequencing methods for:
    • DNA,
    • RNA,
    • microRNA,
    • gene fragments for 16S ribosomal RNA .

variants of cooperation: contract research, project, consortium

type of services: analysis, devices for rent, expertises